Methylene Blue
MB · Methylthioninium chloride · Tetramethylthionine chloride
Mitochondrial electron carrier studied for cognitive enhancement and neuroprotection
Typical Dose
1 mg/kg once daily
Route
Oral
Frequency
Once daily
Cycle
Ongoing or cycled
Overview
Methylene Blue (MB) is a synthetic phenothiazine compound with a long history in medicine. Modern research has revealed its remarkable properties as a mitochondrial electron carrier, antioxidant, and neuroprotective agent. MB can accept electrons from NADH and donate them to cytochrome c in the mitochondrial electron transport chain, effectively bypassing Complex I and III dysfunction.
Key Benefits
Mechanism of Action
Methylene Blue acts as a redox cycling compound and alternative electron carrier in the mitochondrial electron transport chain. At low doses, it enhances mitochondrial respiration by shuttling electrons between NADH and cytochrome c, increasing ATP production. It also inhibits monoamine oxidase (MAO) and acetylcholinesterase, contributing to its neuroprotective and cognitive effects.
Dosing Protocol
Typical Dose
1 mg/kg once daily
Dose Range
0.5-4 mg/kg/day
Frequency
Once daily
Route
Oral capsule
Cycle Length
Ongoing or cycled
CLINICAL NOTES
Note: urine will turn blue/green. This is normal and harmless. Higher doses may cause paradoxical oxidative stress. Avoid with serotonergic medications.
Side Effects & Safety
IMPORTANT DISCLAIMER
This information is for educational purposes only. Always consult a qualified healthcare provider before starting any peptide protocol. Individual responses vary and medical supervision is recommended.
Research References
Common Stacks
Methylene blue enhances mitochondrial function and cognitive performance. It pairs well with nootropic and longevity peptides.
Both support mitochondrial electron transport — a synergistic energy and cognitive stack.
Semax + Methylene Blue is a popular cognitive enhancement combination.
Selank's anxiolytic effects complement Methylene Blue's stimulating cognitive benefits.
Frequently Asked Questions
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